Pancreas glucagon immunolocalization in the scorpion mud turtle, kinosternon scorpioides Running title: pancreas glucagon immunolocalization in turtle

José Gomes Pereira, Mayra da Silva Oliveira, Diego Carvalho Viana, Ana Lúcia Abreu-Silva, Ferdinan Almeida Melo

Abstract


Jurará (Kinosternon scorpioides) is a semi-aquatic fresh water turtle. It is a species of the Brazilian fauna in imminent risk of extinction. Thus studies have been carried out to develop its rearing in captivity. In the present study pancreas fragments were used from six adult K. Scorpioides specimens (three males and three females) blocked in paraffin. Semi-seried3μm thick cuts were obtained in a rotating microtome and fixed on previously treated histological slides (sylanized). The pancreas cuts were submitted to the streptavidin-peroxidase technique to detect glucagon antigens present in the pancreas parenchyma of jurará. Quantitative analysis of the slides was made by counting 20 distinct fields containing glucagon immunoreactive cells under an optical microscope with a 40X objective lens. Glucagon immunoreactive cells were identified in the pancreas parenchymaof the mud turtle, and they were more frequent in the pancreatic islets. They were also observed in the exocrine parenchyma and in the epithelial of the pancreatic duct cover. Histological analysis showed that the pancreatic is lets did not present a capsule of conjunctive tissue. Quantitative analysis showed glucagon immunoreactive cells per field an average of cells 21.4 ± 11.4. Glucagon immmunoreactive cells were observed throughout the organ.

 

 


Keywords


Testudinata. Parenchyma. Pancreaticislet. Chelonia.

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References


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DOI: https://doi.org/10.34117/bjdv6n3-166

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