Multivariate selection of nutrient parameters in submerged culture of kluyveromyces marxianus for biosurfactant production / Seleção multivariada de parâmetros nutricionais em cultivo submerso de kluyveromyces marxianus para produção de biossurfactante

Claudia Eugênia Castro Bravo, Regiane Strapazzon, Roberta Castro Martins, Adir Silvério Cembranel, Ellen Porto Pinto

Abstract


Surfactants are compounds that decrease surface tension and are produced by chemical or biological synthesis. Biosurfactants are produced by the metabolism of microorganisms and have advantages regarding the use of chemicals, mainly due to the low toxicity and biodegradability. Initially a fractional factorial design 2(6-2) was applied to study the effect and interactions of concentrations of different nutrients on the production of biosurfactants by yeast Kluyveromyces marxianus (CCT-3172). Aliquots of 1% (v/v) of microbial inoculum were transferred to the culture media prepared according to the experimental design and incubated on a shaker table at 200 RPM and with a temperature control at 28ºC±2 for 144 hours. The biosurfactant production was verified by the emulsification index (E24) following the methodology of Cooper and Goldenberg (1987). Based on the experimental results, the yeast K. marxianus (CCT-3172) demonstrated potential for biosurfactant production, evidenced by the emulsifying index in fermentation medium. With the application of factorial design, it was concluded that to maximize the biosurfactant production process, the ammonium sulfate and glycerol must be absent from the fermentation medium to eliminate its negative effects in the process. In a next step, the objective is to apply the response surface methodology to optimize biosurfactant production.


Keywords


Environmental microbiology, Culture médium, Emulsifying index, Vegetable oils, Yeast.

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DOI: https://doi.org/10.34117/bjdv6n3-090

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