Atividade citotóxica e anti-inflamatória do extrato glicólico de Camellia sinensis (L.) Kuntze em macrófagos (RAW 264.7) estimulados por LPS / Cytotoxic and antiinflamatory activity of the glycolic extract of Camellia sinensis (L.) Kuntze in LPS-stimulated machrophages (RAW 264.7)

Karen Cristiane Higa, Adeline Lacerda Jorjão, Simone Aparecida Biazzi de Lapena, Everton Tadeu Prado, Fernanda Malagutti Tomé, Andreia Ferreira Diniz Cortelli, Viviane Borio Conceição, Priscila Ebram de Miranda, Luciane Dias de Oliveira

Abstract


Plant extracts can be a source of diverse biologic activities, as anti-inflammatory action, which is an interesting characteristic for mouthwashes, toothpastes and intracanal medication. This study aimed to evaluate cytotoxic and anti-inflammatory activity of Camellia sinensis (L.) Kuntze (green tea) glycolic extract in mouse macrophages (RAW 264.7).  Cytotoxic activity was measured by the metabolic activity of MTT test, macrophages were distributed in 96 wells and exposed to 11 serial dilutions of each extract (200 mg/mL to 0.20 mg/mL). After 5 min and 24 h of contact, cell viability was assessed. Then, the extract at concentrations of 3.13 mg / mL and 12.5 mg / mL, were chosen to verify antiinflammatory activity. For this, after exposure time of 5 min or 24 h to the extract, supernatants of LPS-stimulated RAW 264.7 cultures were collected to quantify pro-inflammatory cytokines IL-1β and TNF-α by immunoenzymatic test (ELISA). The results were evaluated with statistical analyses (ANOVA and Turkey test), with p ≤ 0.05. Results: In MTT test green tea promoted increase cell viability in all concentrations at 5 min. The cell viability was greater than 100% in concentration of 0,20mg/mL to 12,5 mg/mL, at the time of exposure of 24h. The concentration of 12.5 mg/mL was the highest concentration less cytotoxic. The extract showed anti-inflammatory potential evidenced by the production decrease of IL-1β and TNF-α with better results at a concentration of 12.5 mg/mL for both exposure times. These results indicate promising immunomodulatory features of green tea. Therefore, this plant extract showed to be an interesting alternative to be inserted in medical or oral products or even as a source of active compounds.


Keywords


Glycolic extract. Anti-inflammatory. Camellia sinensis. Cytotoxic. Macrophages RAW

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DOI: https://doi.org/10.34119/bjhrv3n1-085

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