Preparation and evaluation of the pentavalent antimony-quercetin complex (SbV-QUE) / Preparação e avaliação do complexo antimonial pentavalente-quercetina (SbV-QUE)

Authors

  • Joyelanne Kaline Chagas Souza
  • Valcilaine Teixeira Barbosa
  • Joyanne Cristine Chagas Souza
  • Janaína Barros de Menezes
  • Luciano Aparecido Meireles Grillo
  • Camila Braga Dornelas

DOI:

https://doi.org/10.34119/bjhrv2n4-074

Keywords:

leishmaniasis, antimonial, quercetin

Abstract

Leishmaniasis is a parasitic disease that has the first choice of treatment medications with antimony as active ingredient. These characteristics justify the search for new compounds with easy administration. In this context, there are quercetin, flavonoid leishmanicidal with chelation power, enabling complexation with metalloids, such as antimony. Thereby, this research aims to prepare and evaluate the complex antimony-quercetin (SbV) in solution and powder form. Precursor materials were quercetin anhydrous (methanolic solution) and potassium hexahydroxoantimonate (V) (aqueous solution), in 1: 1 ratio. After the formation of the complex SbV-QUE SOL (solution), it was dried by rotoevaporation, lyophilization or spray drying. For characterization of SbV-QUE SOL, spectrofluorimetry and UV-Vis spectrophotometry were utilized. In addition, for SbV-QUE powder, X-ray diffraction (XRD), energy dispersive X-Ray analysis (EDX), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA) and fluorescence microscopy were made. In SbV-QUE SOL, an increase of fluorescence and the bathochromic shift in UV-Vis were observed, demonstrating the formation of the complex. In SbV-QUE powder, a difference of crystallinity between precursors and products was perceived, by XRD analysis. Analyzing DX results, a presence of antimony was observed in the samples. FT-IR indicated the binding site of the complex. Finally, the same thermal profile for the powdered complexes was observed, in addition to the micrographs elucidating the surface morphologies of quercetin and SbV-QUE powder. This confirms the characteristic of post-drying fluorescence. Therefore, the formation of the complex was evidenced, which is essential for further investigation of its use, aiming at leishmaniasis treatment.

References

BERLIER, et al. Stabilization of quercetin flavonoid in MCM-41 mesoporous silica: positive effect of surface functionalization. Journal of Colloid and Interface Science, v. 393, p. 109–118, 2013.

BORGES, K. C. Pitanga (Eugenia uniflora) desidratada por atomização e liofilização: características físico-químicas, compostos bioativos e efeito sobre a longevidade, estresse oxidativo e neurotoxicidade induzida em modelos in vivo Caenorhabditis elegans. Tese (Doutorado em engenharia química) - Departamento de Engenharia Química, Universidade Federal Do Rio Grande do Norte, Rio Grande do Norte, 2015.

BRAZIL. Ministério da Saúde. Manual de Vigilância e Controle da Leishmaniose Visceral. Departamento de Vigilância Epidemiológica. 1ed., Brasília: Ministério da Saúde, 2006. P. 120. Disponível em: <http://bvsms.saude.gov.br/bvs/publicacoes/manual_vigilancia_controle_leishmaniose_visceral.pdf>. Acesso em: 01 de janeiro, 2019.

DUOMO, Z et al. Polymorph control of antimony white (Sb2O3) prepared by hydrometallurgy method. Transactions of Nonferrous Metals Society of China. v.7, n.4, p.118-122, 1997.

FICARRA, R. et al. Study of flavonoids/?-cyclodextrins inclusion complexes by NMR, FT-IR, DSC, X-ray investigation. Journal of Pharmaceutical and Biomedical Analysis, v. 29, n. 6, p. 1005-1014, 2002.

LUNA, F. M. T. et al. Rapid assessment of total and polycyclic aromatic contents. Environmental Monitoring and Assessment, v. 188, p. 215, 2016.

MENEZES, J. et al. Layered double hydroxides (LDHs) as carrier of antimony aimed for improving leishmaniasis chemotherapy. Applied Clay Science, v. 91–92, p. 127–134, 2014.

MUZITANO, M.F. et al. Quercitrin: An Antileishmanial Flavonoid Glycoside From Kalanchoe pinnata. Planta Medica, v. 72, p. 81–83, 2006.

NEVES et al. Parasitologia Humana. 13ª Ed. Atheneu Rio, 2016.

PRALHAD, T.; RAJENDRAKUMAR, K. Study of freeze-dried quercetin–cyclodextrin binary systems by DSC, FT-IR, X-ray diffraction and SEM analysis. Journal of Pharmaceutical and Biomedical Analysis, v. 34, n. 2, p. 333-339, 2004.

ROJAS, C. et al. High sensitivity adsorptive stripping voltammetric method for antimony(III) determination in the presence of quercetin-5?-sulfonic acid. Substituent effect on sensitivity. Sensors and Actuators B: Chemical, v. 185, p. 560-567, 2013.

SANNA, D. et al. Behavior Of The Potential Antitumor VIVO Complexes Formed By Flavonoid Ligands. 2. Characterization Of Sulfonate Derivatives Of Quercetin And Morin, Interaction With The Bioligands Of The Plasma And Preliminary Biotransformation Studies. Journal of Inorganic Biochemistry, v. 153, p. 167-177, 2015.

TASDEMIR, D. et al. Antitrypanosomal And Antileishmanial Activities Of Flavonoids And Their Analogues: In Vitro, In Vivo, Structure-Activity Relationship Studies. Antimicrobial Agents and Chemotherapy, v. 50, p. 1352-1364, 2006.

TONG, L. et al. Sb(III)–Quercetin Complex:Synthesis, Characterization And Antioxidant Activity. Life Science Journal, v. 13, n. 7, p. 127-133, 2016.

VIEIRA, F. A. Estudos Sobre O Comportamento Do Antimoniato De Meglumina No Corpo Humano E Em Macacos Rhesus. Tese (Doutorado em Química) – Pontifícia Universidade Católica Do Rio De Janeiro - Puc-Rio, Rio de Janeiro, 2008.

VILA-NOVA, N.S. et al. Leishmanicidal and cholinesterase inhibiting acti-vities of phenolic compounds of Dimorphandra gardneriana and Platymiscium loribundum, native plants from Caatinga biome. Pesquisa Veterinária Brasileira, v. 32, n. 11, p. 1164-1168, 2012.

VISWANATHAN, P. SRIRAM, V. YOGEESWARAN, G. Sensitive spectrophotometric assay for 3-hydroxy-substituted flavonoids, based on their binding with molybdenum, antimony, or bismuth. Journal of Agricultural and Food Chemistry, v. 48, n. 7, p. 2802-2806, 2000.

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Published

2019-06-04

How to Cite

SOUZA, J. K. C.; BARBOSA, V. T.; SOUZA, J. C. C.; MENEZES, J. B. de; GRILLO, L. A. M.; DORNELAS, C. B. Preparation and evaluation of the pentavalent antimony-quercetin complex (SbV-QUE) / Preparação e avaliação do complexo antimonial pentavalente-quercetina (SbV-QUE). Brazilian Journal of Health Review, [S. l.], v. 2, n. 4, p. 3091–3103, 2019. DOI: 10.34119/bjhrv2n4-074. Disponível em: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/2106. Acesso em: 28 mar. 2024.

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Original Papers